Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen
Brigham and Women's Hospital · Howard Hughes Medical Institute · +4 more institutions
Abstract
HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally…
Citation impact
- FWCI
- 50.27
- Percentile
- 100%
- References
- 35
Authors
8- ALAbraham L. BrassCorresponding
Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute, Center for Systems Biology
- DMDerek M. Dykxhoorn
Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute, Center for Systems Biology
- YBYair Benita
Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute, Center for Systems Biology
- NYNan Yan
Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute, Center for Systems Biology
- AEAlan Engelman
Brigham and Women's Hospital, Howard Hughes Medical Institute, Harvard University, Massachusetts General Hospital, Dana-Farber Cancer Institute, Center for Systems Biology
Topics & keywords
- Biology
- RNA interference
- Viral life cycle
- Computational biology
- Small interfering RNA
- Host (biology)
- Gene
- RNA