CRACM1 Is a Plasma Membrane Protein Essential for Store-Operated Ca 2+ Entry
University of Hawaiʻi at Mānoa · Beth Israel Deaconess Medical Center · +3 more institutions
Abstract
Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC…
Citation impact
- FWCI
- 35.08
- Percentile
- 100%
- References
- 15
Authors
11- MVMonika VigCorresponding
University of Hawaiʻi at Mānoa, Beth Israel Deaconess Medical Center, University of Hawaii System, Harvard University, Queen's Medical Center
- CPChristine Peinelt
University of Hawaiʻi at Mānoa, Beth Israel Deaconess Medical Center, University of Hawaii System, Harvard University, Queen's Medical Center
- ABAndreas Beck
University of Hawaiʻi at Mānoa, Beth Israel Deaconess Medical Center, University of Hawaii System, Harvard University, Queen's Medical Center
- DLDana L. Koomoa
University of Hawaiʻi at Mānoa, Beth Israel Deaconess Medical Center, University of Hawaii System, Harvard University, Queen's Medical Center
- DRDania Rabah
University of Hawaiʻi at Mānoa, Beth Israel Deaconess Medical Center, University of Hawaii System, Harvard University, Queen's Medical Center
Topics & keywords
- Gene knockdown
- RNA interference
- Cell biology
- Intracellular
- Chemistry
- Protein subunit
- Patch clamp
- Biology