Cytoplasmic 5′-3′ exonuclease Xrn1p is also a genome-wide transcription factor in yeast

The 5' to 3' exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore, the same study showed that the all tested decaysome components are also nuclear proteins that bind to the 5' region of a number of genes. In the present work, we show that disruption of Xrn1 activity preferentially…

• RF
  Rappaport Foundation
• EC
  European Commission
• GV
  Generalitat Valenciana

  Award: PROMETEO 2011/088
• IS
  Israel Science Foundation