Complete Replication of Hepatitis C Virus in Cell Culture
Scripps Research Institute · Howard Hughes Medical Institute · +3 more institutions
Abstract
Many aspects of the hepatitis C virus (HCV) life cycle have not been reproduced in cell culture, which has slowed research progress on this important human pathogen. Here, we describe a full-length HCV genome that replicates and produces virus particles that are infectious in cell culture (HCVcc). Replication of HCVcc was robust, producing nearly 10(5) infectious units per milliliter within 48 hours. Virus particles were filterable and neutralized with a monoclonal antibody against the viral glycoprotein E2. Viral entry was dependent on cellular expression of a putative HCV receptor, CD81. HCVcc replication was inhibited by interferon-alpha and by several HCV-specific antiviral compounds, suggesting that this…
Citation impact
- FWCI
- 81.74
- Percentile
- 100%
- References
- 24
Authors
11- BDBrett D. Lindenbach
Scripps Research Institute, Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Rockefeller University
- MJMatthew J. Evans
Scripps Research Institute, Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Rockefeller University
- AJAndrew J. Syder
Scripps Research Institute, Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Rockefeller University
- BWBenno Wölk
Scripps Research Institute, Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Rockefeller University
- TLTimothy L. Tellinghuisen
Scripps Research Institute, Howard Hughes Medical Institute, Center for Cancer Research, Massachusetts Institute of Technology, Rockefeller University
Topics & keywords
- Virology
- Hepatitis C virus
- Biology
- Virus
- Viral replication
- Monoclonal antibody
- In vitro
- Interferon
- Responsible consumption and production