Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome
Salk Institute for Biological Studies · Plant Gene Expression Center · +4 more institutions
Abstract
Functional analysis of a genome requires accurate gene structure information and a complete gene inventory. A dual experimental strategy was used to verify and correct the initial genome sequence annotation of the reference plant Arabidopsis. Sequencing full-length cDNAs and hybridizations using RNA populations from various tissues to a set of high-density oligonucleotide arrays spanning the entire genome allowed the accurate annotation of thousands of gene structures. We identified 5817 novel transcription units, including a substantial amount of antisense gene transcription, and 40 genes within the genetically defined centromeres. This approach resulted in completion of approximately 30% of the Arabidopsis…
Citation impact
- FWCI
- 30.58
- Percentile
- 100%
- References
- 42
Authors
70- KYKayoko YamadaCorresponding
Salk Institute for Biological Studies, Plant Gene Expression Center, Center for Genomic Science, University of Pennsylvania, Stanford University
- JLJun LimCorresponding
Salk Institute for Biological Studies, Plant Gene Expression Center, Center for Genomic Science, University of Pennsylvania, Stanford University
- JMJoseph M. Dale
Salk Institute for Biological Studies, Plant Gene Expression Center, Center for Genomic Science, University of Pennsylvania, Stanford University
- HCHuaming Chen
Salk Institute for Biological Studies, Plant Gene Expression Center, Center for Genomic Science, University of Pennsylvania, Stanford University
- PSPaul Shinn
Salk Institute for Biological Studies, Plant Gene Expression Center, Center for Genomic Science, University of Pennsylvania, Stanford University
Topics & keywords
- Arabidopsis
- Genome
- Biology
- Gene
- Computational biology
- Genome project
- Genetics
- Gene Annotation