Mitochondrial α-Ketoglutarate Dehydrogenase Complex Generates Reactive Oxygen Species
Cornell University · University of Maryland, Baltimore · +1 more institution
Abstract
Mitochondria-produced reactive oxygen species (ROS) are thought to contribute to cell death caused by a multitude of pathological conditions. The molecular sites of mitochondrial ROS production are not well established but are generally thought to be located in complex I and complex III of the electron transport chain. We measured H(2)O(2) production, respiration, and NADPH reduction level in rat brain mitochondria oxidizing a variety of respiratory substrates. Under conditions of maximum respiration induced with either ADP or carbonyl cyanide p-trifluoromethoxyphenylhydrazone,alpha-ketoglutarate supported the highest rate of H(2)O(2) production. In the absence of ADP or in the presence of rotenone, H(2)O(2)…
Citation impact
- FWCI
- 9.64
- Percentile
- 100%
- References
- 78
Authors
7Topics & keywords
- Mitochondrion
- Dihydrolipoamide dehydrogenase
- Biochemistry
- Pyruvate dehydrogenase complex
- Reactive oxygen species
- Dehydrogenase
- Respiratory chain
- Biology