Promotion of Reprogramming to Ground State Pluripotency by Signal Inhibition
Wellcome/MRC Cambridge Stem Cell Institute · University of Cambridge
Abstract
Induced pluripotent stem (iPS) cells are generated from somatic cells by genetic manipulation. Reprogramming entails multiple transgene integrations and occurs apparently stochastically in rare cells over many days. Tissue stem cells may be subject to less-stringent epigenetic restrictions than other cells and might therefore be more amenable to deprogramming. We report that brain-derived neural stem (NS) cells acquire undifferentiated morphology rapidly and at high frequency after a single round of transduction with reprogramming factors. However, critical attributes of true pluripotency--including stable expression of endogenous Oct4 and Nanog, epigenetic erasure of X chromosome silencing in female cells,…
Citation impact
- FWCI
- 31.83
- Percentile
- 100%
- References
- 38
Authors
6- JSJosé SilvaCorresponding
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
- OBOrnella Barrandon
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
- JNJennifer Nichols
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
- JKJitsutaro Kawaguchi
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
- TWThorold W. Theunissen
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
Topics & keywords
- Biology
- Reprogramming
- Induced pluripotent stem cell
- Homeobox protein NANOG
- Cell biology
- SOX2
- Stem cell
- KLF4