Evidence Based Selection of Housekeeping Genes
University Medical Center Groningen · University of Groningen
Abstract
For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes (reference or internal control genes) is required. It is known that commonly used housekeeping genes (e.g. ACTB, GAPDH, HPRT1, and B2M) vary considerably under different experimental conditions and therefore their use for normalization is limited. We performed a meta-analysis of 13,629 human gene array samples in order to identify the most stable expressed genes. Here we show novel candidate housekeeping genes (e.g. RPS13, RPL27, RPS20 and OAZ1) with enhanced stability among a multitude of different cell types and varying experimental conditions. None of the commonly used housekeeping genes were…
Citation impact
- FWCI
- 9.02
- Percentile
- 100%
- References
- 16
Authors
10- HJHendrik J.M. de Jonge
University Medical Center Groningen, University of Groningen
- RSRudolf S.N. Fehrmann
University of Groningen, University Medical Center Groningen
- ESEveline S.J.M. de Bont
University Medical Center Groningen, University of Groningen
- RMRobert M.W. Hofstra
University of Groningen, University Medical Center Groningen
- FGFrans Gerbens
University of Groningen, University Medical Center Groningen
Topics & keywords
- Housekeeping gene
- Gene
- Biology
- Reference genes
- Genetics
- Housekeeping
- Computational biology
- Candidate gene
- Life in Land