articleMolecular EcologyApr 1, 2012Closed access

Towards next‐generation biodiversity assessment using DNA metabarcoding

Centre National de la Recherche Scientifique · Université Joseph Fourier · +4 more institutions

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Abstract

Virtually all empirical ecological studies require species identification during data collection. DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample containing entire organisms or from a single environmental sample containing degraded DNA (soil, water, faeces, etc.). It can be implemented for both modern and ancient environmental samples. The availability of next-generation sequencing platforms and the ecologists' need for high-throughput taxon identification have facilitated the emergence of DNA metabarcoding. The potential power of DNA metabarcoding as it is implemented today is limited mainly by its dependency on PCR and by the considerable investment…

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