Hierarchical Action and Inhibition of Plant Dicer-Like Proteins in Antiviral Defense
Centre National de la Recherche Scientifique · Oregon State University · +2 more institutions
Abstract
The mechanisms underlying induction and suppression of RNA silencing in the ongoing plant-virus arms race are poorly understood. We show here that virus-derived small RNAs produced by Arabidopsis Dicer-like 4 (DCL4) program an effector complex conferring antiviral immunity. Inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2. Accordingly, inactivating both DCL2 and DCL4 was necessary and sufficient to restore systemic infection of a suppressor-deficient virus. The effects of DCL2 were overcome by increasing viral dosage in inoculated leaves, but this could not surmount additional, non-cell autonomous effects of DCL4 specifically preventing viral unloading from…
Citation impact
- FWCI
- 75.71
- Percentile
- 100%
- References
- 18
Authors
6- ADAngélique Deleris
Centre National de la Recherche Scientifique, Oregon State University, Institut de Biologie Moléculaire des Plantes, Biocom
- JGJavier Gallego‐Bartolomé
Centre National de la Recherche Scientifique, Oregon State University, Institut de Biologie Moléculaire des Plantes, Biocom
- JBJinsong Bao
Centre National de la Recherche Scientifique, Oregon State University, Institut de Biologie Moléculaire des Plantes, Biocom
- KDKristin D. Kasschau
Centre National de la Recherche Scientifique, Oregon State University, Institut de Biologie Moléculaire des Plantes, Biocom
- JCJames C. Carrington
Centre National de la Recherche Scientifique, Oregon State University, Institut de Biologie Moléculaire des Plantes, Biocom
Topics & keywords
- Biology
- Dicer
- RNA silencing
- Argonaute
- Effector
- Gene silencing
- Trans-acting siRNA
- RNA