Phosphatidylserine-dependent ingestion of apoptotic cells promotes TGF-β1 secretion and the resolution of inflammation
University of Colorado Hospital · University of Colorado Anschutz Medical Campus · +3 more institutions
Abstract
Ingestion of apoptotic cells in vitro by macrophages induces TGF-beta1 secretion, resulting in an anti-inflammatory effect and suppression of proinflammatory mediators. Here, we show in vivo that direct instillation of apoptotic cells enhanced the resolution of acute inflammation. This enhancement appeared to require phosphatidylserine (PS) on the apoptotic cells and local induction of TGF-beta1. Working with thioglycollate-stimulated peritonea or LPS-stimulated lungs, we examined the effect of apoptotic cell uptake on TGF-beta1 induction. Viable or opsonized apoptotic human Jurkat T cells, or apoptotic PLB-985 cells, human monomyelocytes that do not express PS during apoptosis, failed to induce TGF-beta1. PS…
Citation impact
- FWCI
- 18.81
- Percentile
- 100%
- References
- 55
Authors
3- MNMai-Lan N. HuynhCorresponding
University of Colorado Hospital, University of Colorado Anschutz Medical Campus, University of Colorado Denver, University of Colorado Health
- VAValerie A. Fadok
National Jewish Health, University of Colorado Denver
- PMPeter M. Henson
University of Colorado Denver, National Jewish Health
Topics & keywords
- Phosphatidylserine
- Apoptosis
- Proinflammatory cytokine
- Inflammation
- Jurkat cells
- Efferocytosis
- Chemokine
- Cell biology