Antigen-loaded MR1 tetramers define T cell receptor heterogeneity in mucosal-associated invariant T cells
The University of Melbourne · Peter Doherty Institute · +6 more institutions
Abstract
Mucosal-associated invariant T cells (MAIT cells) express a semi-invariant T cell receptor (TCR) α-chain, TRAV1-2-TRAJ33, and are activated by vitamin B metabolites bound by the major histocompatibility complex (MHC)-related class I-like molecule, MR1. Understanding MAIT cell biology has been restrained by the lack of reagents to specifically identify and characterize these cells. Furthermore, the use of surrogate markers may misrepresent the MAIT cell population. We show that modified human MR1 tetramers loaded with the potent MAIT cell ligand, reduced 6-hydroxymethyl-8-D-ribityllumazine (rRL-6-CH₂OH), specifically detect all human MAIT cells. Tetramer(+) MAIT subsets were predominantly CD8(+) or…
Citation impact
- FWCI
- 17.07
- Percentile
- 100%
- References
- 56
Authors
20- RRRangsima Reantragoon
The University of Melbourne, Peter Doherty Institute
- AJAlexandra J. Corbett
The University of Melbourne, Peter Doherty Institute
- IGIsaac G. Sakala
Washington University in St. Louis
- NANicholas A. Gherardin
The University of Melbourne, Peter MacCallum Cancer Centre, Peter Doherty Institute
- JBJohn B. Furness
The University of Melbourne, Peter Doherty Institute
Topics & keywords
- T-cell receptor
- CD8
- Biology
- Major histocompatibility complex
- Population
- T cell
- Cytotoxic T cell
- Antigen