Global mapping of binding sites for Nrf2 identifies novel targets in cell survival response through ChIP-Seq profiling and network analysis
University of British Columbia · Johns Hopkins University · +1 more institution
Abstract
The Nrf2 (nuclear factor E2 p45-related factor 2) transcription factor responds to diverse oxidative and electrophilic environmental stresses by circumventing repression by Keap1, translocating to the nucleus, and activating cytoprotective genes. Nrf2 responses provide protection against chemical carcinogenesis, chronic inflammation, neurodegeneration, emphysema, asthma and sepsis in murine models. Nrf2 regulates the expression of a plethora of genes that detoxify oxidants and electrophiles and repair or remove damaged macromolecules, such as through proteasomal processing. However, many direct targets of Nrf2 remain undefined. Here, mouse embryonic fibroblasts (MEF) with either constitutive nuclear…
Citation impact
- FWCI
- 13.61
- Percentile
- 100%
- References
- 65
Authors
11- DMDeepti MalhotraCorresponding
University of British Columbia, Johns Hopkins University, Child and Family Research Institute
- ÉPÉlodie Portales-Casamar
University of British Columbia, Johns Hopkins University, Child and Family Research Institute
- ASAnju Singh
University of British Columbia, Johns Hopkins University, Child and Family Research Institute
- SSSiddhartha Srivastava
University of British Columbia, Johns Hopkins University, Child and Family Research Institute
- DJDavid J. Arenillas
University of British Columbia, Johns Hopkins University, Child and Family Research Institute
Topics & keywords
- Biology
- Transcription factor
- KEAP1
- Chromatin immunoprecipitation
- Microarray analysis techniques
- Gene expression profiling
- ChIP-sequencing
- Cell biology
- Good health and well-being