Comprehensive Epitope Analysis of Human Immunodeficiency Virus Type 1 (HIV-1)-Specific T-Cell Responses Directed against the Entire Expressed HIV-1 Genome Demonstrate Broadly Directed Responses, but No Correlation to Viral Load
Harvard University · Massachusetts General Hospital · +3 more institutions
Abstract
Cellular immune responses play a critical role in the control of human immunodeficiency virus type 1 (HIV-1); however, the breadth of these responses at the single-epitope level has not been comprehensively assessed. We therefore screened peripheral blood mononuclear cells (PBMC) from 57 individuals at different stages of HIV-1 infection for virus-specific T-cell responses using a matrix of 504 overlapping peptides spanning all expressed HIV-1 proteins in a gamma interferon-enzyme-linked immunospot (Elispot) assay. HIV-1-specific T-cell responses were detectable in all study subjects, with a median of 14 individual epitopic regions targeted per person (range, 2 to 42), and all 14 HIV-1 protein subunits were…
Citation impact
- FWCI
- 20.61
- Percentile
- 100%
- References
- 52
Authors
20- MMMarylyn M. Addo
Harvard University, Massachusetts General Hospital
- XGXu G. Yu
Harvard University, Massachusetts General Hospital
- ARAlmas Rathod
Harvard University, Massachusetts General Hospital
- DCDani Cohen
Lemuel Shattuck Hospital, Fenway Health
- RLRobert L. Eldridge
Harvard University, Massachusetts General Hospital
Topics & keywords
- ELISPOT
- Biology
- Epitope
- Virology
- Immune system
- Virus
- Peripheral blood mononuclear cell
- Immunology
- Good health and well-being