Uric Acid Stimulates Monocyte Chemoattractant Protein-1 Production in Vascular Smooth Muscle Cells Via Mitogen-Activated Protein Kinase and Cyclooxygenase-2
The University of Melbourne · Heidelberg Repatriation Hospital · +3 more institutions
Abstract
Previous studies have reported that uric acid stimulates vascular smooth muscle cell (VSMC) proliferation in vitro. We hypothesized that uric acid may also have direct proinflammatory effects on VSMCs. Crystal- and endotoxin-free uric acid was found to increase VSMC monocyte chemoattractant protein-1 (MCP-1) expression in a time- and dose-dependent manner, peaking at 24 hours. Increased mRNA and protein expression occurred as early as 3 hours after uric acid incubation and was partially dependent on posttranscriptional modification of MCP-1 mRNA. In addition, uric acid activated the transcription factors nuclear factor-kappaB and activator protein-1, as well as the MAPK signaling molecules ERK p44/42 and p38,…
Citation impact
- FWCI
- 10.50
- Percentile
- 100%
- References
- 38
Authors
11- JKJohn KanellisCorresponding
The University of Melbourne, Heidelberg Repatriation Hospital, The University of Texas at Austin, Baewha Women's University, Seoul Women's University
- SWSusumu Watanabe
The University of Melbourne, Heidelberg Repatriation Hospital, The University of Texas at Austin, Baewha Women's University, Seoul Women's University
- JLJinhua Li
The University of Melbourne, Heidelberg Repatriation Hospital, The University of Texas at Austin, Baewha Women's University, Seoul Women's University
- DKDuk‐Hee Kang
The University of Melbourne, Heidelberg Repatriation Hospital, The University of Texas at Austin, Baewha Women's University, Seoul Women's University
- PLPing Li
The University of Melbourne, Heidelberg Repatriation Hospital, The University of Texas at Austin, Baewha Women's University, Seoul Women's University
Topics & keywords
- Proinflammatory cytokine
- Uric acid
- Vascular smooth muscle
- p38 mitogen-activated protein kinases
- Cyclooxygenase
- Protein kinase A
- Internal medicine
- MAPK/ERK pathway