Human neural stem cells differentiate and promote locomotor recovery in spinal cord-injured mice
Salk Institute for Biological Studies · University of California, Irvine · +1 more institution
Abstract
We report that prospectively isolated, human CNS stem cells grown as neurospheres (hCNS-SCns) survive, migrate, and express differentiation markers for neurons and oligodendrocytes after long-term engraftment in spinal cord-injured NOD-scid mice. hCNS-SCns engraftment was associated with locomotor recovery, an observation that was abolished by selective ablation of engrafted cells by diphtheria toxin. Remyelination by hCNS-SCns was found in both the spinal cord injury NOD-scid model and myelin-deficient shiverer mice. Moreover, electron microscopic evidence consistent with synapse formation between hCNS-SCns and mouse host neurons was observed. Glial fibrillary acidic protein-positive astrocytic…
Citation impact
- FWCI
- 11.62
- Percentile
- 100%
- References
- 39
Authors
8- BCB. CummingsCorresponding
Salk Institute for Biological Studies, University of California, Irvine, StemCells (United States)
- NUNobuko Uchida
Salk Institute for Biological Studies, University of California, Irvine, StemCells (United States)
- STStanley Tamaki
Salk Institute for Biological Studies, University of California, Irvine, StemCells (United States)
- DLDesirée L. Salazar
Salk Institute for Biological Studies, University of California, Irvine, StemCells (United States)
- MJMitra J. Hooshmand
Salk Institute for Biological Studies, University of California, Irvine, StemCells (United States)
Topics & keywords
- Remyelination
- Spinal cord injury
- Neurosphere
- Diphtheria toxin
- Myelin
- Neural stem cell
- Stem cell
- Spinal cord