The Vegetative Vacuole Proteome of Arabidopsis thaliana Reveals Predicted and Unexpected Proteins[W]
University of California, Riverside · Plant (United States)
Abstract
Vacuoles play central roles in plant growth, development, and stress responses. To better understand vacuole function and biogenesis we have characterized the vegetative vacuolar proteome from Arabidopsis thaliana. Vacuoles were isolated from protoplasts derived from rosette leaf tissue. Total purified vacuolar proteins were then subjected either to multidimensional liquid chromatography/tandem mass spectrometry or to one-dimensional SDS-PAGE coupled with nano-liquid chromatography/tandem mass spectrometry (nano-LC MS/MS). To ensure maximum coverage of the proteome, a tonoplast-enriched fraction was also analyzed separately by one-dimensional SDS-PAGE followed by nano-LC MS/MS. Cumulatively, 402 proteins were…
Citation impact
- FWCI
- 13.10
- Percentile
- 100%
- References
- 89
Authors
6- CCClay Carter
University of California, Riverside, Plant (United States)
- SPSongqin Pan
University of California, Riverside, Plant (United States)
- JZJan Zouhar
University of California, Riverside, Plant (United States)
- ELEmily L. Avila
University of California, Riverside, Plant (United States)
- TGThomas Girke
University of California, Riverside, Plant (United States)
Topics & keywords
- Vacuole
- Proteome
- Biology
- Arabidopsis thaliana
- Arabidopsis
- Biogenesis
- Cell biology
- Membrane protein