Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung

The lung hosts multiple populations of macrophages and dendritic cells, which play a crucial role in lung pathology. The accurate identification and enumeration of these subsets are essential for understanding their role in lung pathology. Flow cytometry is a mainstream tool for studying the immune system. However, a systematic flow cytometric approach to identify subsets of macrophages and dendritic cells (DCs) accurately and consistently in the normal mouse lung has not been described. Here we developed a panel of surface markers and an analysis strategy that accurately identify all known populations of macrophages and DCs, and their precursors in the lung during steady-state conditions and bleomycin-induced…

• NI
  National Institutes of Health

  Awards: HL108795, HL071643, AR050250, AR054796, AI092490, ES013995, ES015024