Dysfunction of Mitochondria in Human Skeletal Muscle in Type 2 Diabetes

Skeletal muscle is strongly dependent on oxidative phosphorylation for energy production. Because the insulin resistance of skeletal muscle in type 2 diabetes and obesity entails dysregulation of the oxidation of both carbohydrate and lipid fuels, the current study was undertaken to examine the potential contribution of perturbation of mitochondrial function. Vastus lateralis muscle was obtained by percutaneous biopsy during fasting conditions from lean (n = 10) and obese (n = 10) nondiabetic volunteers and from volunteers with type 2 diabetes (n = 10). The activity of rotenone-sensitive NADH:O(2) oxidoreductase, reflecting the overall activity of the respiratory chain, was measured in a mitochondrial fraction…

• UO
  University of Pittsburgh

  Award: M01RR00056
• LM
  Lomonosov Moscow State University
• NI
  National Institutes of Health

  Award: DK49200
• NI
  National Institute of Diabetes and Digestive and Kidney Diseases