The influence of soil pH on the diversity, abundance and transcriptional activity of ammonia oxidizing archaea and bacteria
University of Aberdeen · Mote Marine Laboratory · +2 more institutions
Abstract
Autotrophic ammonia oxidation occurs in acid soils, even though laboratory cultures of isolated ammonia oxidizing bacteria fail to grow below neutral pH. To investigate whether archaea possessing ammonia monooxygenase genes were responsible for autotrophic nitrification in acid soils, the community structure and phylogeny of ammonia oxidizing bacteria and archaea were determined across a soil pH gradient (4.9-7.5) by amplifying 16S rRNA and amoA genes followed by denaturing gradient gel electrophoresis (DGGE) and sequence analysis. The structure of both communities changed with soil pH, with distinct populations in acid and neutral soils. Phylogenetic reconstructions of crenarchaeal 16S rRNA and amoA genes…
Citation impact
- FWCI
- 38.89
- Percentile
- 100%
- References
- 52
Authors
4Topics & keywords
- Archaea
- Biology
- Temperature gradient gel electrophoresis
- Microcosm
- Ammonia monooxygenase
- Soil microbiology
- 16S ribosomal RNA
- Autotroph
- Life in Land