BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
University of Geneva · SIB Swiss Institute of Bioinformatics
Abstract
MOTIVATION: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50. RESULTS: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO. AVAILABILITY AND IMPLEMENTATION: Software implemented in Python and datasets available for download from http://busco.ezlab.org. CONTACT: evgeny.zdobnov@unige.ch SUPPLEMENTARY INFORMATION: Supplementary data are…
Citation impact
- FWCI
- 232.04
- Percentile
- 100%
- References
- 16
Authors
5- FAFelipe A. Simão
University of Geneva, SIB Swiss Institute of Bioinformatics
- RMRobert M. Waterhouse
University of Geneva, SIB Swiss Institute of Bioinformatics
- PIPanagiotis Ioannidis
University of Geneva, SIB Swiss Institute of Bioinformatics
- EVEvgenia V. Kriventseva
University of Geneva, SIB Swiss Institute of Bioinformatics
- EMEvgeny M. ZdobnovCorresponding
University of Geneva, SIB Swiss Institute of Bioinformatics
Topics & keywords
- Python (programming language)
- Annotation
- Benchmarking
- Computer science
- Software
- Genome
- Genomics
- Gene Annotation