Mechanisms to suppress multipolar divisions in cancer cells with extra centrosomes
Harvard University · Dana-Farber Cancer Institute · +7 more institutions
Abstract
Multiple centrosomes in tumor cells create the potential for multipolar divisions that can lead to aneuploidy and cell death. Nevertheless, many cancer cells successfully divide because of mechanisms that suppress multipolar mitoses. A genome-wide RNAi screen in Drosophila S2 cells and a secondary analysis in cancer cells defined mechanisms that suppress multipolar mitoses. In addition to proteins that organize microtubules at the spindle poles, we identified novel roles for the spindle assembly checkpoint, cortical actin cytoskeleton, and cell adhesion. Using live cell imaging and fibronectin micropatterns, we found that interphase cell shape and adhesion pattern can determine the success of the subsequent…
Citation impact
- FWCI
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- Percentile
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- References
- 76
Authors
7- MKMijung KwonCorresponding
Harvard University, Dana-Farber Cancer Institute
- SASusana A. Godinho
Harvard University, Dana-Farber Cancer Institute
- NSNamrata S. Chandhok
Harvard University, Dana-Farber Cancer Institute
- NJNeil J. Ganem
Harvard University, Dana-Farber Cancer Institute
- AAAmmar Azioune
Centre National de la Recherche Scientifique, Biologie cellulaire et Cancer, Institut Curie
Topics & keywords
- Centrosome
- Biology
- Mitosis
- Cell biology
- Cancer cell
- Multipolar spindles
- Kinesin
- Spindle pole body
- Good health and well-being