Real-Time Monitoring of Cell Apoptosis and Drug Screening Using Fluorescent Light-Up Probe with Aggregation-Induced Emission Characteristics
National University of Singapore · Hong Kong University of Science and Technology · +4 more institutions
Abstract
Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This…
Citation impact
- FWCI
- 20.08
- Percentile
- 100%
- References
- 63
Authors
6- HSHaibin ShiCorresponding
National University of Singapore
- RTRyan T. K. Kwok
Hong Kong University of Science and Technology, Institute of Molecular Functional Materials
- JLJianzhao Liu
Hong Kong University of Science and Technology, Institute of Molecular Functional Materials
- BXBengang Xing
Nanyang Technological University
- BZBen Zhong Tang
Hong Kong University of Science and Technology, Institute of Molecular Functional Materials, Institute of Materials Research and Engineering, South China University of Technology
Topics & keywords
- Chemistry
- Fluorescence
- Aggregation-induced emission
- Apoptosis
- Biophysics
- Drug
- Fluorescent light
- Biochemistry
- Good health and well-being