Osteogenesis Associates With Inflammation in Early-Stage Atherosclerosis Evaluated by Molecular Imaging In Vivo
Brigham and Women's Hospital · Harvard University · +1 more institution
Abstract
BACKGROUND: Arterial calcification is associated with cardiovascular events; however, mechanisms of calcification in atherosclerosis remain obscure. METHODS AND RESULTS: We tested the hypothesis that inflammation promotes osteogenesis in atherosclerotic plaques using in vivo molecular imaging in apolipoprotein E-/- mice (20 to 30 weeks old, n=35). A bisphosphonate-derivatized near-infrared fluorescent imaging agent (excitation 750 nm) visualized osteogenic activity that was otherwise undetectable by x-ray computed tomography. Flow cytometry validated the target specifically in osteoblast-like cells. A spectrally distinct near-infrared fluorescent nanoparticle (excitation 680 nm) was coinjected to…
Citation impact
- FWCI
- 15.64
- Percentile
- 100%
- References
- 43
Authors
9- EAElena AïkawaCorresponding
Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital
- MNMatthias Nahrendorf
Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital
- JFJose‐Luiz Figueiredo
Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital
- FKFilip K. Świrski
Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital
- TSTimur Shtatland
Brigham and Women's Hospital, Harvard University, Massachusetts General Hospital
Topics & keywords
- In vivo
- Alkaline phosphatase
- Calcification
- Pathology
- Osteopontin
- Medicine
- Inflammation
- Flow cytometry
- Good health and well-being