reviewAnnual Review of Physical ChemistryApr 4, 2012Closed access

Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging

THTaekjip HaPTPhilip Tinnefeld

Howard Hughes Medical Institute · University of Illinois Urbana-Champaign · +1 more institution

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Abstract

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important elements of the ongoing technical revolution to reveal biochemical and cellular processes in unprecedented clarity and precision. Demands placed on the photophysical properties of the fluorophores are stringent and drive the choice of appropriate probes. Such fluorophores are not simple light bulbs of a certain color and brightness but instead have their own "personalities" regarding spectroscopic parameters, redox properties, size, water solubility, photostability, and several other factors. Here, we review the photophysics of fluorescent probes, both organic fluorophores and fluorescent proteins, used in applications such…

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Authors

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Topics & keywords

Topics
Keywords
  • Fluorescence
  • Förster resonance energy transfer
  • Nanotechnology
  • Molecule
  • Single-molecule experiment
  • Chemistry
  • Fluorophore
  • Materials science
UN Sustainable Development Goals
  • Clean water and sanitation
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