A hyperactive piggyBac transposase for mammalian applications
Wellcome Sanger Institute · Howard Hughes Medical Institute · +1 more institution
Abstract
DNA transposons have been widely used for transgenesis and insertional mutagenesis in various organisms. Among the transposons active in mammalian cells, the moth-derived transposon piggyBac is most promising with its highly efficient transposition, large cargo capacity, and precise repair of the donor site. Here we report the generation of a hyperactive piggyBac transposase. The active transposition of piggyBac in multiple organisms allowed us to screen a transposase mutant library in yeast for hyperactive mutants and then to test candidates in mouse ES cells. We isolated 18 hyperactive mutants in yeast, among which five were also hyperactive in mammalian cells. By combining all mutations, a total of 7 aa…
Citation impact
- FWCI
- 12.37
- Percentile
- 100%
- References
- 45
Authors
5- KYKosuke YusaCorresponding
Wellcome Sanger Institute
- LZLiqin Zhou
Howard Hughes Medical Institute, Johns Hopkins University
- MAMeng Amy Li
Howard Hughes Medical Institute, Johns Hopkins University, Wellcome Sanger Institute
- ABAllan Bradley
Wellcome Sanger Institute
- NLNancy L. Craig
Howard Hughes Medical Institute, Johns Hopkins University
Topics & keywords
- Transposase
- Biology
- Transposable element
- P element
- Insertional mutagenesis
- Genetics
- Mutant
- Mutagenesis