Emergent Properties of Reduced-Genome Escherichia coli
Friedrich-Loeffler-Institut · University of Wisconsin–Madison · +5 more institutions
Abstract
With the use of synthetic biology, we reduced the Escherichia coli K-12 genome by making planned, precise deletions. The multiple-deletion series (MDS) strains, with genome reductions up to 15%, were designed by identifying nonessential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving good growth profiles and protein production. Genome reduction also led to unanticipated beneficial properties: high electroporation efficiency and accurate propagation of recombinant genes and plasmids that were unstable in other strains. Eradication of stress-induced transposition evidently stabilized the MDS genomes and provided some of the new properties.
Citation impact
- FWCI
- 17.93
- Percentile
- 100%
- References
- 17
Authors
13- GPGyörgy PósfaiCorresponding
Friedrich-Loeffler-Institut, University of Wisconsin–Madison, HUN-REN Szegedi Biológiai Kutatóközpont, Scarab Genomics (United States), Institute of Biochemistry, Energy Center of Wisconsin
- GPGuy Plunkett
Friedrich-Loeffler-Institut, University of Wisconsin–Madison, HUN-REN Szegedi Biológiai Kutatóközpont, Scarab Genomics (United States), Institute of Biochemistry, Energy Center of Wisconsin
- TFTamás Fehér
Friedrich-Loeffler-Institut, University of Wisconsin–Madison, HUN-REN Szegedi Biológiai Kutatóközpont, Scarab Genomics (United States), Institute of Biochemistry, Energy Center of Wisconsin
- DFDavid Frisch
Friedrich-Loeffler-Institut, University of Wisconsin–Madison, HUN-REN Szegedi Biológiai Kutatóközpont, Scarab Genomics (United States), Institute of Biochemistry, Energy Center of Wisconsin
- GMGünther M. Keil
Friedrich-Loeffler-Institut, University of Wisconsin–Madison, HUN-REN Szegedi Biológiai Kutatóközpont, Scarab Genomics (United States), Institute of Biochemistry, Energy Center of Wisconsin
Topics & keywords
- Genome
- Escherichia coli
- Plasmid
- Biology
- Transposition (logic)
- Gene
- Genetics
- Mobile genetic elements