Genome-wide mapping of Polycomb target genes unravels their roles in cell fate transitions
Centre for Research in Intensive Care · University of Copenhagen
Abstract
The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find that Polycomb-Repressive Complex 1 (PRC1), PRC2, and tri-methylated histone H3K27 co-occupy >1000 silenced genes with a strong functional bias for embryonic development and cell fate decisions. We functionally identify 40 genes derepressed in human embryonic fibroblasts depleted of the PRC2 components (EZH2, EED, SUZ12) and the PRC1 component, BMI-1. Interestingly, several markers of osteogenesis, adipogenesis, and…
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- References
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Authors
5- APAdrian P. BrackenCorresponding
Centre for Research in Intensive Care
- NDNikolaj Dietrich
Centre for Research in Intensive Care
- DPDiego Pasini
Centre for Research in Intensive Care
- KHKlaus Hansen
Centre for Research in Intensive Care
- KHKristian Helin
University of Copenhagen, Centre for Research in Intensive Care
Topics & keywords
- PRC2
- Biology
- Embryonic stem cell
- Polycomb-group proteins
- Chromatin
- Gene
- Histone
- Genetics