Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic water
University of Kaiserslautern · Natural History Museum · +4 more institutions
Abstract
Sequencing of ribosomal DNA clone libraries amplified from environmental DNA has revolutionized our understanding of microbial eukaryote diversity and ecology. The results of these analyses have shown that protist groups are far more genetically heterogeneous than their morphological diversity suggests. However, the clone library approach is labour-intensive, relatively expensive, and methodologically biased. Therefore, even the most intensive rDNA library analyses have recovered only small samples of much larger assemblages, indicating that global environments harbour a vast array of unexplored biodiversity. High-throughput parallel tag 454 sequencing offers an unprecedented scale of sampling for molecular…
Citation impact
- FWCI
- 29.82
- Percentile
- 100%
- References
- 61
Authors
7Topics & keywords
- Anoxic waters
- DNA sequencing
- Environmental DNA
- Biology
- Computational biology
- DNA
- Ecology
- Environmental science
- Life below water