Primer3—new capabilities and interfaces
Heidelberg University · National Center for Biotechnology Information · +4 more institutions
Abstract
Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for successful PCR, and for over a decade, the open-source Primer3 software has been widely used for primer design, often in high-throughput genomics applications. It has also been incorporated into numerous publicly available software packages and web services. During this period, we have greatly expanded Primer3's functionality. In this article, we describe Primer3's current capabilities, emphasizing recent improvements. The…
Citation impact
- FWCI
- 367.40
- Percentile
- 100%
- References
- 51
Authors
7- AUAndreas Untergasser
Heidelberg University, National Center for Biotechnology Information, Bioinformatics Institute, DKFZ-ZMBH Alliance, University of Tartu, Institute of Molecular and Cell Biology
- ICIoana Cutcutache
Heidelberg University, National Center for Biotechnology Information, Bioinformatics Institute, DKFZ-ZMBH Alliance, University of Tartu, Institute of Molecular and Cell Biology
- TKTriinu Kõressaar
Heidelberg University, National Center for Biotechnology Information, Bioinformatics Institute, DKFZ-ZMBH Alliance, University of Tartu, Institute of Molecular and Cell Biology
- JYJian Ye
Heidelberg University, National Center for Biotechnology Information, Bioinformatics Institute, DKFZ-ZMBH Alliance, University of Tartu, Institute of Molecular and Cell Biology
- BCBrant C. Faircloth
Heidelberg University, National Center for Biotechnology Information, Bioinformatics Institute, DKFZ-ZMBH Alliance, University of Tartu, Institute of Molecular and Cell Biology
Topics & keywords
- Biology
- Primer (cosmetics)
- Computational biology
- Software
- Modular design
- Genotyping
- Genomics
- Polymerase chain reaction
- Industry, innovation and infrastructure