The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote
Fred Hutch Cancer Center · Office of Basic Energy Sciences · +6 more institutions
Abstract
The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity. To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome-wide chromatin structure analysis in a higher eukaryote. We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues. Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions,…
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Authors
12- DSDirk Schübeler
Fred Hutch Cancer Center, Office of Basic Energy Sciences, Friedrich Miescher Institute
- DMDavid M. MacAlpine
Howard Hughes Medical Institute, Fred Hutch Cancer Center, Massachusetts Institute of Technology
- DSDavid Scalzo
Fred Hutch Cancer Center, Office of Basic Energy Sciences
- CWC Wirbelauer
Fred Hutch Cancer Center, Friedrich Miescher Institute
- CKCharles Kooperberg
Fred Hutch Cancer Center, Faculty of Public Health
Topics & keywords
- Biology
- Eukaryote
- Histone
- Chromatin
- Genetics
- Histone code
- Histone methyltransferase
- Euchromatin