articleThe Journal of ImmunologyNov 1, 2002BRONZE OA

Expression of Programmed Death 1 Ligands by Murine T Cells and APC

TYTomohide YamazakiHAHisaya AkibaHIHideyuki IwaiHMHironori MatsudaMAMami Aoki

Juntendo University · Tokyo Medical and Dental University · +2 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

Programmed death 1 (PD-1) is a new member of the CD28/CTLA-4 family, which has been implicated in the maintenance of peripheral tolerance. Two ligands for PD-1, namely, B7-H1 (PD-L1) and B7-DC (PD-L2), have recently been identified as new members of the B7 family but their expression at the protein level remains largely unknown. To characterize the expression of B7-H1 and B7-DC, we newly generated an anti-mouse B7-H1 mAb (MIH6) and an anti-mouse B7-DC mAb (TY25). MIH6 and TY25 immunoprecipitated a single molecule of 43 and 42 kDa from the lysate of B7-H1 and B7-DC transfectants, respectively. Flow cytometric analysis revealed that B7-H1 was broadly expressed on the surface of mouse tumor cell lines while the…

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Topics & keywords

Topics
Keywords
  • CD40
  • CD28
  • Molecular biology
  • Biology
  • T cell
  • Cell biology
  • Antigen-presenting cell
  • Chemistry
UN Sustainable Development Goals
  • Good health and well-being
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