Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification
University of Ottawa · Institute for Neurodegenerative Disorders · +1 more institution
Abstract
A rapid means of assessing reproductive status in rodents is useful not only in the study of reproductive dysfunction but is also required for the production of new mouse models of disease and investigations into the hormonal regulation of tissue degeneration (or regeneration) following pathological challenge. The murine reproductive (or estrous) cycle is divided into 4 stages: proestrus, estrus, metestrus, and diestrus. Defined fluctuations in circulating levels of the ovarian steroids 17-β-estradiol and progesterone, the gonadotropins luteinizing and follicle stimulating hormones, and the luteotropic hormone prolactin signal transition through these reproductive stages. Changes in cell typology within the…
Citation impact
- FWCI
- 16.61
- Percentile
- 100%
- References
- 27
Authors
4- ACAshleigh C. McLeanCorresponding
University of Ottawa, Institute for Neurodegenerative Disorders, Ottawa Institute of Systems Biology
- NVNicolas Valenzuela
University of Ottawa, Institute for Neurodegenerative Disorders
- SFStephen Fai
Institute for Neurodegenerative Disorders, University of Ottawa
- SASteffany A. L. Bennett
Institute for Neurodegenerative Disorders, University of Ottawa, Ottawa Institute of Systems Biology
Topics & keywords
- Estrous cycle
- Luteinizing hormone
- Biology
- Hormone
- Prolactin
- Physiology
- Vaginal smear
- Vagina