OligoCalc: an online oligonucleotide properties calculator
Robert H. Lurie Comprehensive Cancer Center of Northwestern University · Northwestern University
Abstract
We developed OligoCalc as a web-accessible, client-based computational engine for reporting DNA and RNA single-stranded and double-stranded properties, including molecular weight, solution concentration, melting temperature, estimated absorbance coefficients, inter-molecular self-complementarity estimation and intra-molecular hairpin loop formation. OligoCalc has a familiar 'calculator' look and feel, making it readily understandable and usable. OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature. Since it first came online in 1997, there have been more than 900,000 accesses of OligoCalc from…
Citation impact
- FWCI
- 12.24
- Percentile
- 100%
- References
- 15
Authors
1Topics & keywords
- Calculator
- Oligonucleotide
- USable
- Biology
- Complementarity (molecular biology)
- Melting curve analysis
- Computer science
- Table (database)