The Transcriptional and Epigenomic Foundations of Ground State Pluripotency
Radboud University Nijmegen · Radboud University Medical Center · +4 more institutions
Abstract
Mouse embryonic stem (ES) cells grown in serum exhibit greater heterogeneity in morphology and expression of pluripotency factors than ES cells cultured in defined medium with inhibitors of two kinases (Mek and GSK3), a condition known as "2i" postulated to establish a naive ground state. We show that the transcriptome and epigenome profiles of serum- and 2i-grown ES cells are distinct. 2i-treated cells exhibit lower expression of lineage-affiliated genes, reduced prevalence at promoters of the repressive histone modification H3K27me3, and fewer bivalent domains, which are thought to mark genes poised for either up- or downregulation. Nonetheless, serum- and 2i-grown ES cells have similar differentiation…
Citation impact
- FWCI
- 37.08
- Percentile
- 100%
- References
- 67
Authors
11- HMHendrik MarksCorresponding
Radboud University Nijmegen, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences
- TKTüzer Kalkan
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
- RMRoberta Menafra
Radboud University Nijmegen, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences
- SDSergey Denissov
Radboud University Nijmegen, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences
- KDKenneth D. Jones
Wellcome/MRC Cambridge Stem Cell Institute, University of Cambridge
Topics & keywords
- Biology
- Epigenomics
- State (computer science)
- Computational biology
- Genetics
- DNA methylation
- Gene expression
- Gene