Histone methyltransferases G9a and GLP form heteromeric complexes and are both crucial for methylation of euchromatin at H3-K9
Kyoto University · The University of Tokyo · +1 more institution
Abstract
Histone H3 Lys 9 (H3-K9) methylation is a crucial epigenetic mark for transcriptional silencing. G9a is the major mammalian H3-K9 methyltransferase that targets euchromatic regions and is essential for murine embryogenesis. There is a single G9a-related methyltransferase in mammals, called GLP/Eu-HMTase1. Here we show that GLP is also important for H3-K9 methylation of mouse euchromatin. GLP-deficiency led to embryonic lethality, a severe reduction of H3-K9 mono- and dimethylation, the induction of Mage-a gene expression, and HP1 relocalization in embryonic stem cells, all of which were phenotypes of G9a-deficiency. Furthermore, we show that G9a and GLP formed a stoichiometric heteromeric complex in a wide…
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Authors
10- MTMakoto TachibanaCorresponding
Kyoto University, The University of Tokyo, Kumamoto University
- JUJun Ueda
Kyoto University, The University of Tokyo, Kumamoto University
- MFMikiko Fukuda
Kyoto University, The University of Tokyo, Kumamoto University
- NTNaoki Takeda
Kyoto University, The University of Tokyo, Kumamoto University
- TOTsutomu Ohta
Kyoto University, The University of Tokyo, Kumamoto University
Topics & keywords
- Biology
- Methyltransferase
- Histone methyltransferase
- Histone H3
- Euchromatin
- Epigenetics
- Histone methylation
- Methylation