Guidelines for the use and interpretation of assays for monitoring autophagy
University of Michigan · Hebrew University of Jerusalem · +167 more institutions
Abstract
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the…
Citation impact
- FWCI
- 340.06
- Percentile
- 100%
- References
- 898
Authors
1270Topics & keywords
- Autophagy
- Autophagosome
- Biology
- Cell biology
- Multicellular organism
- Phagosome
- ATG8
- Biogenesis