Comparative Analysis of Proteome and Transcriptome Variation in Mouse
University of California, Los Angeles · Pacific Northwest National Laboratory · +5 more institutions
Abstract
The relationships between the levels of transcripts and the levels of the proteins they encode have not been examined comprehensively in mammals, although previous work in plants and yeast suggest a surprisingly modest correlation. We have examined this issue using a genetic approach in which natural variations were used to perturb both transcript levels and protein levels among inbred strains of mice. We quantified over 5,000 peptides and over 22,000 transcripts in livers of 97 inbred and recombinant inbred strains and focused on the 7,185 most heritable transcripts and 486 most reliable proteins. The transcript levels were quantified by microarray analysis in three replicates and the proteins were quantified…
Citation impact
- FWCI
- 23.97
- Percentile
- 100%
- References
- 46
Authors
26- AGAnatole GhazalpourCorresponding
University of California, Los Angeles
- BJBrian J. Bennett
University of California, Los Angeles
- VPVladislav Petyuk
Pacific Northwest National Laboratory, Environmental Molecular Sciences Laboratory
- LDLuz D. Orozco
University of California, Los Angeles
- RHRaffi Hagopian
University of California, Los Angeles
Topics & keywords
- Biology
- Proteome
- Gene
- Transcriptome
- Genetics
- Inbred strain
- Proteomics
- Genome
Funding
- UDU.S. Department of EnergyAwards: 76RL01830, AC05-76RL01830, Contract DE-AC05-76RL01830, DE-AC05-76RL01830, DE-AC05
- AHAmerican Heart Association
- BBattelleAwards: AC05-76RL01830, DE-AC05, DE-AC05-76RL01830
- NINational Institutes of HealthAwards: HG002536, HL28481, GM07104, NRSA T32, R01 NS050148, HL30568, DE-AC05-76RL01830
- PNPacific Northwest National LaboratoryAwards: DE-AC05-76RL01830, 76RL01830