Collagen Degradation by Host-derived Enzymes during Aging
University of Trieste · Universidade de São Paulo · +4 more institutions
Abstract
Incompletely infiltrated collagen fibrils in acid-etched dentin are susceptible to degradation. We hypothesize that degradation can occur in the absence of bacteria. Partially demineralized collagen matrices (DCMs) prepared from human dentin were stored in artificial saliva. Control specimens were stored in artificial saliva containing proteolytic enzyme inhibitors, or pure mineral oil. We retrieved them at 24 hrs, 90 and 250 days to examine the extent of degradation of DCM. In the 24-hour experimental and 90- and 250-day control specimens, we observed 5- to 6-microm-thick layers of DCM containing banded collagen fibrils. DCMs were almost completely destroyed in the 250-day experimental specimens, but not when…
Citation impact
- FWCI
- 20.41
- Percentile
- 100%
- References
- 30
Authors
7- DPD.H. PashleyCorresponding
University of Trieste, Universidade de São Paulo, Hokkaido University, Augusta University, University of Hong Kong
- FRFranklin R. Tay
University of Trieste, Universidade de São Paulo, Hokkaido University, Augusta University, University of Hong Kong
- CYCincia Yiu
University of Trieste, Universidade de São Paulo, Hokkaido University, Augusta University, University of Hong Kong
- MHMakoto Hashimoto
University of Trieste, Universidade de São Paulo, Hokkaido University, Augusta University, University of Hong Kong
- LBLorenzo Breschi
University of Trieste, Universidade de São Paulo, Hokkaido University, Augusta University, University of Hong Kong
Topics & keywords
- Dentin
- Saliva
- Degradation (telecommunications)
- Chemistry
- Enzyme
- Protease
- Matrix metalloproteinase
- Demineralization
- Clean water and sanitation