articleClinical ChemistryFeb 27, 2009BRONZE OA

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

Queen Mary University of London · University College London Hospitals NHS Foundation Trust · +9 more institutions

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Abstract

BACKGROUND: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of sufficient experimental detail in many publications, which impedes a reader's ability to evaluate critically the quality of the results presented or to repeat the experiments. CONTENT: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency. MIQE is a set of guidelines that describe the minimum information necessary for…

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