articleJournal of Clinical MicrobiologySep 1, 2005Closed access

Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii

SGSergio G. BartualHSHarald SeifertCHCorinna HipplerMAMaria Angeles Dominguez LuzónHWHilmar Wisplinghoff

Universitat de Miguel Hernández d'Elx · University of Cologne · +1 more institution

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Abstract

In this study a multilocus sequence typing (MLST) scheme for Acinetobacter baumannii was developed and evaluated by using 40 clinical A. baumannii isolates recovered from outbreaks in Spanish and German hospitals during the years 1990 to 2001, as well as isolates from other European hospitals and two DSMZ reference strains of A. baumannii. For comparison, two isolates of Acinetobacter species 13 (sensu Tjernberg and Ursing), two clinical isolates, and three DSMZ strains of A. calcoaceticus (both belonging to the A. calcoaceticus-A. baumannii complex) were also investigated. Primers were designed for conserved regions of housekeeping genes, and 305- to 513-bp internal fragments of seven such genes-gltA, gyrB,…

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Topics & keywords

Topics
Keywords
  • Multilocus sequence typing
  • Acinetobacter baumannii
  • Biology
  • Housekeeping gene
  • Typing
  • Genetics
  • Microbiology
  • Molecular epidemiology
UN Sustainable Development Goals
  • Good health and well-being
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