A practical guide to small angle X‐ray scattering (SAXS) of flexible and intrinsically disordered proteins
European Molecular Biology Laboratory · European Molecular Biology Laboratory
Abstract
Small-angle X-ray scattering (SAXS) is a biophysical method to study the overall shape and structural transitions of biological macromolecules in solution. SAXS provides low resolution information on the shape, conformation and assembly state of proteins, nucleic acids and various macromolecular complexes. The technique also offers powerful means for the quantitative analysis of flexible systems, including intrinsically disordered proteins (IDPs). Here, the basic principles of SAXS are presented, and profits and pitfalls of the characterization of multidomain flexible proteins and IDPs using SAXS are discussed from the practical point of view. Examples of the synergistic use of SAXS with high resolution…
Citation impact
- FWCI
- 19.37
- Percentile
- 100%
- References
- 97
Authors
2Topics & keywords
- Small-angle X-ray scattering
- Intrinsically disordered proteins
- Macromolecule
- Scattering
- Small-angle scattering
- Characterization (materials science)
- Low resolution
- Crystallography