Worldwide Epidemiology of Neutralizing Antibodies to Adeno‐Associated Viruses

Recombinant adeno-associated viruses (AAVs) have unique gene-transfer properties that speak to their potential as carriers for gene therapy or vaccine applications. However, the presence of neutralizing antibodies to AAV as a result of previous exposure can significantly limit effective gene transfer. In this study, we obtained 888 human serum samples from healthy volunteers in 10 countries around the world. Samples were assayed for neutralizing antibodies to AAV1, AAV2, AAV7, and AAV8, as well as to a novel, structurally distinct AAV vector, rh32.33, in an in vitro transduction inhibition assay. Our data revealed that neutralizing antibodies to AAV2 were the most prevalent antibodies in all regions, followed…

• IA
  International AIDS Vaccine Initiative
• G
  GlaxoSmithKline
• UO
  University of Pennsylvania
• VR
  Vlaamse regering
• NI
  National Institutes of Health
• IO
  Institute of Genetics