Trp-dependent auxin biosynthesis in Arabidopsis : involvement of cytochrome P450s CYP79B2 and CYP79B3
Boston University · Salk Institute for Biological Studies · +2 more institutions
Abstract
The plant hormone auxin regulates many aspects of plant growth and development. Although several auxin biosynthetic pathways have been proposed, none of these pathways has been precisely defined at the molecular level. Here we provide in planta evidence that the two Arabidopsis cytochrome P450s, CYP79B2 and CYP79B3, which convert tryptophan (Trp) to indole-3-acetaldoxime (IAOx) in vitro, are critical enzymes in auxin biosynthesis in vivo. IAOx is thus implicated as an important intermediate in auxin biosynthesis. Plants overexpressing CYP79B2 contain elevated levels of free auxin and display auxin overproduction phenotypes. Conversely, cyp79B2 cyp79B3 double mutants have reduced levels of IAA and show growth…
Citation impact
- FWCI
- —
- Percentile
- —
- References
- 37
Authors
9- YZYunde ZhaoCorresponding
Boston University, Salk Institute for Biological Studies, Howard Hughes Medical Institute, University of Massachusetts Amherst
- AKAnna K. Hull
Boston University, Salk Institute for Biological Studies, Howard Hughes Medical Institute, University of Massachusetts Amherst
- NRNeeru R. Gupta
Boston University, Salk Institute for Biological Studies, Howard Hughes Medical Institute, University of Massachusetts Amherst
- KAKendrick A. Goss
Boston University, Salk Institute for Biological Studies, Howard Hughes Medical Institute, University of Massachusetts Amherst
- JMJosé M. Alonso
Boston University, Salk Institute for Biological Studies, Howard Hughes Medical Institute, University of Massachusetts Amherst
Topics & keywords
- Auxin
- Biology
- Arabidopsis
- Plant hormone
- Mutant
- Biochemistry
- Biosynthesis
- Yucca