Phase transitions of multivalent proteins can promote clustering of membrane receptors

Clustering of proteins into micrometer-sized structures at membranes is observed in many signaling pathways. Most models of clustering are specific to particular systems, and relationships between physical properties of the clusters and their molecular components are not well understood. We report biochemical reconstitution on supported lipid bilayers of protein clusters containing the adhesion receptor Nephrin and its cytoplasmic partners, Nck and N-WASP. With Nephrin attached to the bilayer, multivalent interactions enable these proteins to polymerize on the membrane surface and undergo two-dimensional phase separation, producing micrometer-sized clusters. Dynamics and thermodynamics of the clusters are…

• HH
  Howard Hughes Medical Institute
• WF
  Welch Foundation

  Award: I-1544
• NI
  National Institute of General Medical Sciences

  Award: R01-GM56322