The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans
QB3 · Howard Hughes Medical Institute · +3 more institutions
Abstract
Experimental manipulation of protein abundance in living cells or organisms is an essential strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques for protein expression have been developed in Caenorhabditis elegans, existing tools for conditional disruption of protein function are far more limited. To address this, we have adapted the auxin-inducible degradation (AID) system discovered in plants to enable conditional protein depletion in C. elegans. We report that expression of a modified Arabidopsis TIR1 F-box protein mediates robust auxin-dependent depletion of degron-tagged targets. We document the effectiveness of this system for depletion of nuclear and cytoplasmic…
Citation impact
- FWCI
- 33.00
- Percentile
- 100%
- References
- 59
Authors
4- LZLiangyu ZhangCorresponding
QB3, Howard Hughes Medical Institute, Lawrence Berkeley National Laboratory, University of California, Berkeley
- JDJordan D. Ward
University of California, San Francisco
- ZCZe Cheng
University of California, Berkeley
- AFAbby F. Dernburg
QB3, Howard Hughes Medical Institute, Lawrence Berkeley National Laboratory, University of California, Berkeley
Topics & keywords
- Degron
- Biology
- CRISPR
- Cell biology
- Cas9
- Germline
- Caenorhabditis elegans
- Function (biology)
- Life in Land
Funding
- NSNational Science FoundationAward: P40 OD010440
- UOUniversity of MinnesotaAward: P40 OD010440
- NINational Institutes of HealthAwards: OD010440, P40 OD010440, GM065591
- UOUniversity of California, San Francisco
- NINational Institute of General Medical SciencesAwards: GM065591, P40 OD010440
- OOOffice of Research Infrastructure Programs, National Institutes of Health