A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis
University of Kansas · Kansas State University · +3 more institutions
Abstract
Tumor-derived circulating exosomes, enriched with a group of tumor antigens, have been recognized as a promising biomarker source for cancer diagnosis via a less invasive procedure. Quantitatively pinpointing exosome tumor markers is appealing, yet challenging. In this study, we developed a simple microfluidic approach (ExoSearch) which provides enriched preparation of blood plasma exosomes for in situ, multiplexed detection using immunomagnetic beads. The ExoSearch chip offers a robust, continuous-flow design for quantitative isolation and release of blood plasma exosomes in a wide range of preparation volumes (10 μL to 10 mL). We employed the ExoSearch chip for blood-based diagnosis of ovarian cancer by…
Citation impact
- FWCI
- 18.52
- Percentile
- 100%
- References
- 55
Authors
4- ZZZheng Zhao
University of Kansas, Kansas State University
- YYYang Yang
University of Kansas
- YZYong ZengCorresponding
University of Kansas, The University of Kansas Cancer Center
- MHMei HeCorresponding
Kansas State University, The University of Kansas Cancer Center, Cancer Research Center, Johnson Cancer Research Center
Topics & keywords
- Exosome
- Microvesicles
- Ovarian cancer
- Circulating tumor cell
- Biomarker
- Whole blood
- Cancer biomarkers
- Cancer
- Good health and well-being
Funding
- UOUniversity of Kansas
- KIKansas IDeA Network of Biomedical Research ExcellenceAward: P20GM103418
- NINational Institutes of HealthAwards: P20GM103638, P20GM103418
- JCJohnson Cancer Research Center, Kansas State University
- CCCancer Center, University of Kansas
- NCNational Cancer InstituteAward: R21CA186846
- NINational Institute of General Medical SciencesAwards: P20GM103418, P20GM103638