Human cerebral organoids recapitulate gene expression programs of fetal neocortex development
Max Planck Institute for Evolutionary Anthropology · Max Planck Institute of Molecular Cell Biology and Genetics · +8 more institutions
Abstract
Cerebral organoids-3D cultures of human cerebral tissue derived from pluripotent stem cells-have emerged as models of human cortical development. However, the extent to which in vitro organoid systems recapitulate neural progenitor cell proliferation and neuronal differentiation programs observed in vivo remains unclear. Here we use single-cell RNA sequencing (scRNA-seq) to dissect and compare cell composition and progenitor-to-neuron lineage relationships in human cerebral organoids and fetal neocortex. Covariation network analysis using the fetal neocortex data reveals known and previously unidentified interactions among genes central to neural progenitor proliferation and neuronal differentiation. In the…
Citation impact
- FWCI
- 33.28
- Percentile
- 100%
- References
- 39
Authors
15- JGJ. Gray CampCorresponding
Max Planck Institute for Evolutionary Anthropology
- FBFarhath Badsha
Max Planck Institute of Molecular Cell Biology and Genetics
- MFMarta Florio
Max Planck Institute of Molecular Cell Biology and Genetics
- SKSabina Kanton
Max Planck Institute for Evolutionary Anthropology
- TGTobias Gerber
Max Planck Institute for Evolutionary Anthropology
Topics & keywords
- Neocortex
- Organoid
- Biology
- Fetus
- Neuroscience
- Brain development
- Gene expression
- Gene