Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip

A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. This in vitro model replicated results from past animal and human studies, including demonstration that probiotic and antibiotic therapies can suppress villus injury induced by pathogenic bacteria. By ceasing peristalsis-like motions while maintaining luminal flow, lack of epithelial deformation was shown to trigger bacterial overgrowth similar to that observed…

• HU
  Harvard University
• HH
  Hamilton Health Sciences Foundation
• DA
  Defense Advanced Research Projects Agency

  Award: W911NF-12-2-0036
• AR
  Advanced Research Projects Agency
• HW
  Hansjörg Wyss Institute for Biologically Inspired Engineering, Harvard University