A liver-on-a-chip platform with bioprinted hepatic spheroids
Harvard University · Brigham and Women's Hospital · +7 more institutions
Abstract
The inadequacy of animal models in correctly predicting drug and biothreat agent toxicity in humans has resulted in a pressing need for in vitro models that can recreate the in vivo scenario. One of the most important organs in the assessment of drug toxicity is liver. Here, we report the development of a liver-on-a-chip platform for long-term culture of three-dimensional (3D) human HepG2/C3A spheroids for drug toxicity assessment. The bioreactor design allowed for in situ monitoring of the culture environment by enabling direct access to the hepatic construct during the experiment without compromising the platform operation. The engineered bioreactor could be interfaced with a bioprinter to fabricate 3D…
Citation impact
- FWCI
- 34.29
- Percentile
- 100%
- References
- 77
Authors
16- NSNupura S. Bhise
Harvard University, Brigham and Women's Hospital, Massachusetts Institute of Technology, Harvard–MIT Division of Health Sciences and Technology, Innovation Research Center
- VMVijayan Manoharan
Brigham and Women's Hospital, Harvard University, Massachusetts Institute of Technology, Harvard–MIT Division of Health Sciences and Technology, Innovation Research Center
- SMSolange Massa
Harvard University, Brigham and Women's Hospital, Innovation Research Center, Massachusetts Institute of Technology, Universidad de Los Andes, Chile, Harvard–MIT Division of Health Sciences and Technology
- ATAli TamayolCorresponding
Innovation Research Center, Massachusetts Institute of Technology, Harvard–MIT Division of Health Sciences and Technology, Brigham and Women's Hospital, Harvard University
- MGMasoumeh Ghaderi
Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Brigham and Women's Hospital, Innovation Research Center, Harvard University
Topics & keywords
- In vivo
- Spheroid
- Toxicity
- Pharmacology
- In vitro
- Biomedical engineering
- Chemistry
- Biology